
ASEPTIC TECHNIQUE (LAB BENCH)
Lab bench level aseptic technique emphasizes the preparation of a clean workspace, labeling, sterile transfer of liquids, and transfer of a microorganism from solid to liquid culture media. Earners of this credential have aseptic technique knowledge and awareness of creating a clean lab bench.
Earners of this credential have shown mastery of the following tasks:
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Prepare the lab bench surface for aseptic benchwork.
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Handle instruments such as Bunsen burner (or equivalent), striker, inoculating loop, test tubes, serological pipet, pipet pump, and agar plates appropriately
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Aseptically transfer a bacterial colony to a culture tube with broth media.
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Aseptically transfer a volume of sterile broth to a new sterile tube.
GET READY
When you are ready to schedule your credential assessment, ensure that you can complete the tasks listed below:
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Transfer a bacterial colony
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Arrange all materials in efficient locations while transferring
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Make sure a Bunsen burner is lit appropriately and adjust if necessary
COST
For your $20 credential fee, you will be assessed in-person on the tasks listed above and issued a digital credential through Badgr. Our evaluators are available on a flexible schedule.
Credential Alignment
Texas Biomanufacturing Skills Standards
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2.1.2 Personal protection equipment (PPE) such as gloves, eye protection, aprons and respirators are worn as needed
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2.1.4 Appropriate agent(s) and amounts for cleaning are used
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4.6.4 Proper aseptic techniques are followed.
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6.1.2 Microbial growth is identified
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6.1.7 Basic microbiology lab techniques are performed
Washington Skill Standards
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A4 Maintain biological stock cultures
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A6 Prepare biological and/or chemical materials
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B5 Handle and/or maintain biological stock cultures
BACE
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Skill category: Biotechnology
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Demonstrate proper aseptic/sterile technique
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Accurately measure liquids using micropipette and serological pipets
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Use of inoculating loop.
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Use of agar plate media.
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Use of culture tube with broth media.
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Handling of agar plate lid and test tube lids.
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Appropriate use of an inoculating loop to obtain a bacterial colony.
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Measure the volume indicated on a serological pipet (range of 1-9 mL).
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Aseptically transfer the volume to a new sterile tube.