AsepticBanner.png

ASEPTIC TECHNIQUE (LAB BENCH)

Lab bench level aseptic technique emphasizes the preparation of a clean workspace, labeling, sterile transfer of liquids, and transfer of a microorganism from solid to liquid culture media.  Earners of this credential have aseptic technique knowledge and awareness of creating a clean lab bench.

Earners of this credential have shown mastery of the following tasks:​

  • Prepare the lab bench surface for aseptic benchwork.

  • Handle instruments such as Bunsen burner (or equivalent), striker, inoculating loop, test tubes, serological pipet, pipet pump, and agar plates appropriately

  • Aseptically transfer a bacterial colony to a culture tube with broth media.

  • Aseptically transfer a volume of sterile broth to a new sterile tube.

GET READY

   

When you are ready to schedule your credential assessment, ensure that you can complete the tasks listed below: 

  • Transfer a bacterial colony 

  • Arrange all materials in efficient locations while transferring

  • Make sure a Bunsen burner is lit appropriately and adjust if necessary

COST

    

For your $20 credential fee, you will be assessed in-person on the tasks listed above and issued a digital credential through Badgr.  Our evaluators are available on a flexible schedule.

Credential Alignment

Texas Biomanufacturing Skills Standards

  • 2.1.2 Personal protection equipment (PPE) such as gloves, eye protection, aprons and respirators are worn as needed

  • 2.1.4 Appropriate agent(s) and amounts for cleaning are used

  • 4.6.4 Proper aseptic techniques are followed.

  • 6.1.2 Microbial growth is identified

  • 6.1.7 Basic microbiology lab techniques are performed

Washington Skill Standards

  • A4 Maintain biological stock cultures

  • A6 Prepare biological and/or chemical materials

  • B5 Handle and/or maintain biological stock cultures

BACE 

  • Skill category: Biotechnology

    • Demonstrate proper aseptic/sterile technique

    • Accurately measure liquids using micropipette and serological pipets

  • Use of inoculating loop.

  • Use of agar plate media.

  • Use of culture tube with broth media.

  • Handling of agar plate lid and test tube lids.

  • Appropriate use of an inoculating loop to obtain a bacterial colony.

  • Measure the volume indicated on a serological pipet (range of 1-9 mL).

  • Aseptically transfer the volume to a new sterile tube.