ASEPTIC TECHNIQUE (LAB BENCH)
Transfer of sterile solutions and cultures at the lab bench.
Prepare the lab bench surface for aseptic benchwork.
Handle instruments such as Bunsen burner (or equivalent), striker, inoculating loop, test tubes, serological pipet, pipet pump, and agar plates appropriately
Aseptically transfer a bacterial colony to a culture tube with broth media.
Aseptically transfer a volume of sterile broth to a new sterile tube.
Student will be given the task of transferring a bacterial colony.
All materials are located and arranged to be efficient while transferring bacteria.
Bunsen burner is lit appropriately and adjusted if necessary.
Texas Biomanufacturing Skills Standards
2.1.2 Personal protection equipment (PPE) such as gloves, eye protection, aprons and respirators are worn as needed
2.1.4 Appropriate agent(s) and amounts for cleaning are used
4.6.4 Proper aseptic techniques are followed.
6.1.2 Microbial growth is identified
6.1.7 Basic microbiology lab techniques are performed
Washington Skill Standards
A4 Maintain biological stock cultures
A6 Prepare biological and/or chemical materials
B5 Handle and/or maintain biological stock cultures
Skill category: Biotechnology
Demonstrate proper aseptic/sterile technique
Accurately measure liquids using micropipette and serological pipets
Use of inoculating loop.
Use of agar plate media.
Use of culture tube with broth media.
Handling of agar plate lid and test tube lids.
Appropriate use of an inoculating loop to obtain a bacterial colony.
Measure the volume indicated on a serological pipet (range of 1-9 mL).
Aseptically transfer the volume to a new sterile tube.