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ASEPTIC TECHNIQUE (LAB BENCH)

Transfer of sterile solutions and cultures at the lab bench.

Objectives Measured

  • Prepare the lab bench surface for aseptic benchwork.

  • Handle instruments such as Bunsen burner (or equivalent), striker, inoculating loop, test tubes, serological pipet, pipet pump, and agar plates appropriately

  • Aseptically transfer a bacterial colony to a culture tube with broth media.

  • Aseptically transfer a volume of sterile broth to a new sterile tube.

Performance Assessed

  • Student will be given the task of transferring a bacterial colony.

  • All materials are located and arranged to be efficient while transferring bacteria.

  • Bunsen burner is lit appropriately and adjusted if necessary.

Credential Alignment

Texas Biomanufacturing Skills Standards

  • 2.1.2 Personal protection equipment (PPE) such as gloves, eye protection, aprons and respirators are worn as needed

  • 2.1.4 Appropriate agent(s) and amounts for cleaning are used

  • 4.6.4 Proper aseptic techniques are followed.

  • 6.1.2 Microbial growth is identified

  • 6.1.7 Basic microbiology lab techniques are performed

Washington Skill Standards

  • A4 Maintain biological stock cultures

  • A6 Prepare biological and/or chemical materials

  • B5 Handle and/or maintain biological stock cultures

BACE 

  • Skill category: Biotechnology

    • Demonstrate proper aseptic/sterile technique

    • Accurately measure liquids using micropipette and serological pipets

  • Use of inoculating loop.

  • Use of agar plate media.

  • Use of culture tube with broth media.

  • Handling of agar plate lid and test tube lids.

  • Appropriate use of an inoculating loop to obtain a bacterial colony.

  • Measure the volume indicated on a serological pipet (range of 1-9 mL).

  • Aseptically transfer the volume to a new sterile tube.