ASEPTIC TECHNIQUE (LAB BENCH)

Transfer of sterile solutions and cultures at the lab bench.

Objectives Measured

• Prepare the lab bench surface for aseptic benchwork.

• Handle instruments such as Bunsen burner (or equivalent), striker, inoculating loop, test tubes, serological pipet, pipet pump, and agar plates appropriately

• Aseptically transfer a bacterial colony to a culture tube with broth media.

• Aseptically transfer a volume of sterile broth to a new sterile tube.

Performance Assessed

• Student will be given the task of transferring a bacterial colony.

• All materials are located and arranged to be efficient while transferring bacteria.

• Bunsen burner is lit appropriately and adjusted if necessary.

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Credential Alignment​

Texas Biomanufacturing Skills Standards

• 2.1.2 Personal protection equipment (PPE) such as gloves, eye protection, aprons and respirators are worn as needed

• 2.1.4 Appropriate agent(s) and amounts for cleaning are used

• 4.6.4 Proper aseptic techniques are followed.

• 6.1.2 Microbial growth is identified

• 6.1.7 Basic microbiology lab techniques are performed

Washington Skill Standards

• A4 Maintain biological stock cultures

• A6 Prepare biological and/or chemical materials

• B5 Handle and/or maintain biological stock cultures

BACE 

• Skill category: Biotechnology

  • Demonstrate proper aseptic/sterile technique

  • Accurately measure liquids using micropipette and serological pipets

• Use of inoculating loop.

• Use of agar plate media.

• Use of culture tube with broth media.

• Handling of agar plate lid and test tube lids.

• Appropriate use of an inoculating loop to obtain a bacterial colony.

• Measure the volume indicated on a serological pipet (range of 1-9 mL).

• Aseptically transfer the volume to a new sterile tube.

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