SMALL VOLUME METROLOGY
Pipetting volumes from 1 microliter to 25 milliliters
For employers: Individuals with this credential will be able to measure small volumes accurately and operate safely while performing measurement tasks.
Throughout the testing process, the credential earner should wear appropriate Personal Protective Equipment (PPE) and work safely. Volumes of liquid to be measured accurately will range from 1 microliter to 50 milliliters.
Required tasks to earn this credential
Credential earners will be asked to complete the following tasks while being observed by BCSI evaluators:
Determine the appropriate device to use to deliver three different volumes (A, B, C) of liquid into new containers. Multiple aliquots of each volume will be delivered into new tubes. (For example 4 aliquots of Volume A, 4 aliquots of Volume B, etc.)
Performance Assessed
For each volume requested:
Correct instrument is chosen based on the volume of liquid to be delivered.
Correct tip is chosen when necessary.
Interact with the following instruments and equipment appropriately:
Serological pipet
Pipette pump/controller
Micropipette
Micropipet tips
Correct container/tube is chosen for delivering new volume.
Disposal of tip or pipet is done appropriately.
Accurate delivery of the volume into each new tube. (assessed by measuring weight)
Credential Alignment
Washington State
A1. Maintain laboratory and equipment
A3. Operate equipment
Knowledge of equipment operation and procedures for equipment such as pipette-aid, pipettmen, micropipettes, pH meter, centrifuge, scale, autoclave, or spectrophotometer, cleaning spatulas, stir bars and flow hoods.
Knowledge of equipment cleaning procedures and ability to keep equipment in good operating condition.
BACE
Skill Category: Biotechnology Skills
• Accurately measure liquids using micropipettes and serological pipets
Core Skill Standards for Bioscience Technicians
Critical Work Function: Perform measurements / tests / assays
Key Activities
Collect samples according to established procedures and applicable sampling plans
Prepare samples according to established procedures
Follow appropriate test procedures/instructions
Document data & results according to established procedures Interpret and/or analyze data & results as appropriate