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SMALL VOLUME METROLOGY

Pipetting volumes from 1 microliter to 25 milliliters

For employers: Individuals with this credential will be able to measure small volumes accurately and operate safely while performing measurement tasks. 

Throughout the testing process, the credential earner should wear appropriate Personal Protective Equipment (PPE) and work safely.  Volumes of liquid to be measured accurately will range from 1 microliter to 50 milliliters.

Required tasks to earn this credential

Credential earners will be asked to complete the following tasks while being observed by BCSI evaluators:

  1. Determine the appropriate device to use to deliver three different volumes (A, B, C) of liquid into new containers.  Multiple aliquots of each volume will be delivered into new tubes. (For example 4 aliquots of Volume A, 4 aliquots of Volume B, etc.)

Performance Assessed

For each volume requested:

  1. Correct instrument is chosen based on the volume of liquid to be delivered.

  2. Correct tip is chosen when necessary.

  3. Interact with the following instruments and equipment appropriately: 

    1. Serological pipet

    2. Pipette pump/controller

    3. Micropipette

    4. Micropipet tips

  4. Correct container/tube is chosen for delivering new volume.

  5. Disposal of tip or pipet is done appropriately.

  6. Accurate delivery of the volume into each new tube. (assessed by measuring weight) 

Credential Alignment

Washington State

A1. Maintain laboratory and equipment

A3. Operate equipment

Knowledge of equipment operation and procedures for equipment such as pipette-aid, pipettmen, micropipettes, pH meter, centrifuge, scale, autoclave, or spectrophotometer, cleaning spatulas, stir bars and flow hoods.

Knowledge of equipment cleaning procedures and ability to keep equipment in good operating condition.


BACE

Skill Category: Biotechnology Skills
• Accurately measure liquids using micropipettes and serological pipets

Core Skill Standards for Bioscience Technicians

Critical Work Function: Perform measurements / tests / assays 

Key Activities 

  • Collect samples according to established procedures and applicable sampling plans 

  • Prepare samples according to established procedures 

  • Follow appropriate test procedures/instructions 

  • Document data & results according to established procedures Interpret and/or analyze data & results as appropriate